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Restriction fragment‐length polymorphism analysis of 16S rDNA from oral asaccharolytic Eubacterium species amplified by polymerase chain reaction

Identifieur interne : 009890 ( Main/Exploration ); précédent : 009889; suivant : 009891

Restriction fragment‐length polymorphism analysis of 16S rDNA from oral asaccharolytic Eubacterium species amplified by polymerase chain reaction

Auteurs : T. Sato [Japon] ; M. Sato [Japon] ; J. Matsuyama [Japon] ; S. Kalfas [Suède] ; G. Sundqvist [Suède] ; E. Hoshino [Japon]

Source :

Oral Microbiology and Immunology [ 0902-0055 ] ; 1998-02.

RBID : ISTEX:C557F09F248FCFF6193717242DA671FD64288499

English descriptors

Abstract

Restriction fragment‐length polymorphism (RFLP) analysis of 16S rDNA amplified by polymerase chain reaction was used to generate restriction profiles of the type strains of oral asaccharolytic Eubacterium species, that is, Eubacterium brachy, Eubacterium exiguum, Eubacterium lentum, Eubacterium minutum, Eubacterium nodatum, Eubacterium saphenum, Eubacterium timidum and 33 asaccharolytic Eubacterium strains isolated from oral sites. The 16S rRNA gene sequences from isolated genomic DNA samples were amplified by polymerase chain reaction (PCR). PCR products were purified and characterized by single digestions with 7 restriction endonucleases. Among the 7 endonucleases, Hpall was found to discriminate the respective reference strains. Twenty‐three isolates, out of 33, were assigned to one of the reference species, on the basis of their restriction profiles by digestion with Hpall. The remaining 10 isolates could not be assigned to any of the established species and constituted 4 distinct groups, each of which may be a new species.

Url:

https://api.istex.fr/document/C557F09F248FCFF6193717242DA671FD64288499/fulltext/pdf

DOI: 10.1111/j.1399-302X.1998.tb00746.x

Affiliations:

Japon, Suède

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Le document en format XML

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<term>Distinct groups</term>
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<term>Hoshino</term>
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<term>Microbiol</term>
<term>Minutum</term>
<term>Necrotic</term>
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<term>Nodatum</term>
<term>Oral asaccharolytic eubacterium</term>
<term>Oral asaccharolytic eubacterium species</term>
<term>Oral microbiol immunol</term>
<term>Periodontal</term>
<term>Periodontal pocket</term>
<term>Polymerase chain reaction</term>
<term>Predominant obligate anaerobes</term>
<term>Rdna</term>
<term>Reference strains</term>
<term>Respective reference strains</term>
<term>Restriction profiles</term>
<term>Rflp</term>
<term>Rflp analysis</term>
<term>Root canal</term>
<term>Same pattern</term>
<term>Saphenum</term>
<term>Sat0</term>
<term>Sundqvist</term>
<term>Syst bacteriol</term>
<term>Tardum</term>
<term>Timidum</term>
<term>Timidum atcc</term>
<term>Tongue plaque</term>
<term>Type strains</term>
<term>Weightman</term>
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<term>Asaccharolytic eubacterium species</term>
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<term>Bacteriologic evaluation</term>
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<term>Sat0</term>
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<term>Syst bacteriol</term>
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<front><div type="abstract" xml:lang="en">Restriction fragment‐length polymorphism (RFLP) analysis of 16S rDNA amplified by polymerase chain reaction was used to generate restriction profiles of the type strains of oral asaccharolytic Eubacterium species, that is, Eubacterium brachy, Eubacterium exiguum, Eubacterium lentum, Eubacterium minutum, Eubacterium nodatum, Eubacterium saphenum, Eubacterium timidum and 33 asaccharolytic Eubacterium strains isolated from oral sites. The 16S rRNA gene sequences from isolated genomic DNA samples were amplified by polymerase chain reaction (PCR). PCR products were purified and characterized by single digestions with 7 restriction endonucleases. Among the 7 endonucleases, Hpall was found to discriminate the respective reference strains. Twenty‐three isolates, out of 33, were assigned to one of the reference species, on the basis of their restriction profiles by digestion with Hpall. The remaining 10 isolates could not be assigned to any of the established species and constituted 4 distinct groups, each of which may be a new species.</div>
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Restriction fragment‐length polymorphism analysis of 16S rDNA from oral asaccharolytic Eubacterium species amplified by polymerase chain reaction

Restriction fragment‐length polymorphism analysis of 16S rDNA from oral asaccharolytic Eubacterium species amplified by polymerase chain reaction

Source :

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri